| 目的：探討膀胱癌的發(fā)生與P16基因缺失的關(guān)系。方法：采用雙重PCR法對31例膀胱癌組織中P16基因進行檢測。結(jié)果：31例膀胱癌組織中有7例存在P16基因缺失，缺失率為22.6%。結(jié)論：P16基因缺失與某些膀胱癌的發(fā)生存在一定的聯(lián)系。
The deletions of P16 gene detected in bladder cancer by duplex PCR
LI Ze-hui
(Department of Urology,the Second Affiliated Hospital to Kunming Medical College,Kunming,650101)
XU Shao-bin ZHU Jia-you
(Division of Gecomics,Institute of Medical Biology,Chinese Academy of Medical Sciences)
GUO Ping ZHANG Zhong-ji
(Department of Pathology,Kunming Medical College)
SHI Yong-gang
(Department of Urology,the First Afilliated Hospital,Kunming Medical College)
Abstract Purpose:To investigate the deletion of P16 g ene in the bladder cancer.Methods:The P16 gene was analysi s by duplex PCR in 31 bladder cancer tissue samples.Results:The gene deletions were found in seven samples.The deletion ratio of P16 gene was 22.6 per cent.Five cases had deletion in exon 2. One case had deletion in exon 1 and one had deletion in both exon 1 and exon 2.Conclusions:The results suggest that the deletion of P16 gene were associated with some bladder cancer.
Key words Bladder tumor Gene,P16 Polymeruse chain reaction Gene deletion |
